Overview of a Verigene^® Test Cartridge

General description:

• Single use, self-contained, test unit comprised of the following:
  • Reagent pack — single use plastic carrier that creates a hybridization chamber on the surface of the array, houses all of the reagents needed for a single Test, and captures all of the waste materials generated during Test processing.
  • Slide (an array) — glass slide, solid support for the capture oligonucleotides required by the Test.

Capacity:

• Each Test Cartridge is designed for single-use analysis of one sample.
• The typical sample loading volume is 25 µL.

Functionality:

• All of the wet chemistry required to run a test occur inside a Test Cartridge, including:
  • Fragmentation and denaturation of genomic DNA or RNA via on-board sonication.
  • Primary hybridization of genomic DNA or RNA to capture probe oligonucleotides and mediator oligonucleotides under conditions of sufficient stringency. 
  • Washing away of unbound sample nucleic acids and mediator oligonucleotides.
  • Secondary hybridization of gold nanoparticle probes to captured target nucleic acid / mediator oligonucleotide complexes.
  • Washing away of unbound gold nanoparticle probes with oligonucleotides.
  • Signal amplification of gold nanoparticles oligonucleotide probes.
  • Washing away of unreacted signal amplification reagents.